Unknown

Dataset Information

0

RelA inhibits Bacillus subtilis motility and chaining.


ABSTRACT: The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, including virulence, persister cell formation, and biofilm production. In Bacillus subtilis, (p)ppGpp production is primarily determined by the net activity of RelA, a bifunctional (p)ppGpp synthetase/hydrolase, and two monofunctional (p)ppGpp synthetases, YwaC and YjbM. We observe that in B. subtilis, a relA mutant grows exclusively as unchained, motile cells, phenotypes regulated by the alternative sigma factor SigD. Our data indicate that the relA mutant is trapped in a SigD "on" state during exponential growth, implicating RelA and (p)ppGpp levels in the regulation of cell chaining and motility in B. subtilis. Our results also suggest that minor variations in basal (p)ppGpp levels can significantly skew developmental decision-making outcomes.

SUBMITTER: Ababneh QO 

PROVIDER: S-EPMC4288673 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

altmetric image

Publications

RelA inhibits Bacillus subtilis motility and chaining.

Ababneh Qutaiba O QO   Herman Jennifer K JK  

Journal of bacteriology 20141020 1


The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, including virulence, persister cell formation, and biofilm production. In Bacillus subtilis, (p)ppGpp production is primarily determined by the net activity of RelA, a bifunctional (  ...[more]

Similar Datasets

| S-EPMC2998389 | biostudies-literature
| S-EPMC4054197 | biostudies-literature
| S-EPMC3057162 | biostudies-literature
2021-01-20 | GSE164348 | GEO
| S-EPMC6192195 | biostudies-literature
| S-EPMC8316123 | biostudies-literature
2015-01-26 | GSE65272 | GEO
| S-EPMC2911795 | biostudies-literature
| S-EPMC3303961 | biostudies-literature
| S-EPMC10810213 | biostudies-literature