Ontology highlight
ABSTRACT:
SUBMITTER: Suh J
PROVIDER: S-EPMC3305830 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Suh Junghae J An Yoojin Y Tang Benjamin C BC Dempsey Christopher C Huang Feiran F Hanes Justin J
Microscopy research and technique 20111117 5
Using live-cell confocal microscopy and particle tracking technology, the simultaneous transport of intracellular vesicles of the endo-lysosomal pathway and nonviral polyethylenimine (PEI)/DNA nanocomplexes was investigated. Due to potential problems associated with the use of acid-sensitive probes in combination with a gene vector that is hypothesized to buffer the pH of intracellular vesicles, the biological location of PEI/DNA gene vectors was revealed by probing their trafficking in cells ex ...[more]