Ontology highlight
ABSTRACT:
SUBMITTER: Wells NP
PROVIDER: S-EPMC3061257 | biostudies-literature | 2010 Nov
REPOSITORIES: biostudies-literature
Wells Nathan P NP Lessard Guillaume A GA Goodwin Peter M PM Phipps Mary E ME Cutler Patrick J PJ Lidke Diane S DS Wilson Bridget S BS Werner James H JH
Nano letters 20101101 11
We report a method for tracking individual quantum dot (QD) labeled proteins inside of live cells that uses four overlapping confocal volume elements and active feedback once every 5 ms to follow three-dimensional molecular motion. This method has substantial advantages over three-dimensional molecular tracking methods based upon charge-coupled device cameras, including increased Z-tracking range (10 μm demonstrated here), substantially lower excitation powers (15 μW used here), and the ability ...[more]