Dataset Information

Effects of blood products on inflammatory response in endothelial cells in vitro.

ABSTRACT:

Background

Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products.

Methods

The inflammatory response from pre-activated (endotoxin-stimulated) and non-activated endothelial cells as well as neutrophil endothelial transmigration in response to packed red blood cells (PRBC), platelet concentrates (PC) and fresh frozen plasma (FFP) was determined. Baseline inflammatory mediator and lipid concentrations in blood products were evaluated.

Results

Following incubation with all blood products, an increased inflammatory mediator release from endothelial cells was observed. Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells. Inflammatory response of endothelial cells as well as blood product-induced migration of neutrophils through the endothelium was in good agreement with the lipid content of the according blood product.

Conclusion

Within the group of different blood transfusion products both PC and FFP have a high inflammatory potential with regard to activation of endothelial cells. Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured. High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

SUBMITTER: Urner M

PROVIDER: S-EPMC3306413 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Publications

Effects of blood products on inflammatory response in endothelial cells in vitro.

Urner Martin M Herrmann Inge K IK Buddeberg Felix F Schuppli Caroline C Roth Z'graggen Birgit B Hasler Melanie M Schanz Urs U Mehr Manuela M Spahn Donat R DR Beck Schimmer Beatrice B

PloS one 20120316 3

<h4>Background</h4>Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products.<h4>Methods</h4>The inflammatory response from pre-activated (endotoxin-stimulated) and non-activated en ...[more]

PMID: 22438924

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Dataset Information

Effects of blood products on inflammatory response in endothelial cells in vitro.

Background

Methods

Results

Conclusion

Publications

Effects of blood products on inflammatory response in endothelial cells in vitro.

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