Ontology highlight
ABSTRACT:
SUBMITTER: Goodman AL
PROVIDER: S-EPMC3310428 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Goodman Andrew L AL Wu Meng M Gordon Jeffrey I JI
Nature protocols 20111117 12
Insertion sequencing (INSeq) is a method for determining the insertion site and relative abundance of large numbers of transposon mutants in a mixed population of isogenic mutants of a sequenced microbial species. INSeq is based on a modified mariner transposon containing MmeI sites at its ends, allowing cleavage at chromosomal sites 16-17 bp from the inserted transposon. Genomic regions adjacent to the transposons are amplified by linear PCR with a biotinylated primer. Products are bound to mag ...[more]