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Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus.

ABSTRACT: CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, ? = ? = ? = 90.0°.

SUBMITTER: Chen J

PROVIDER: S-EPMC3310551 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature

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Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus.

Chen Jing J Morita Hiroyuki H Kato Ryohei R Noguchi Hiroshi H Sugio Shigetoshi S Abe Ikuro I

Acta crystallographica. Section F, Structural biology and crystallization communications 20120223 Pt 3

CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°. ...[more]

PMID: 22442243

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