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Potential of exogenous cartilage proteoglycan as a new material for cartilage regeneration.


ABSTRACT: BACKGROUND: Although proteoglycan (PG) is one of the major components of cartilage matrices, its biological function is not fully elucidated. METHODS: The objectives of this study were to investigate the proliferation and differentiation of chondrocytes embedded in atelocollagen gel with exogenous cartilage PG (PG-atelocollagen gel) in vitro, and also to evaluate the repair of cartilage defects by PG-atelocollagen gel in vivo. In the in vitro study, rabbit chondrocytes were cultured in the PG-atelocollagen gel. Cell proliferation and mRNA expression levels were measured, and gels were histologically evaluated. In the in vivo study, cultured PG-atelocollagen gel containing chondrocytes were transplanted into full-thickness articular cartilage defects in rabbit knees, and evaluated macroscopically and histologically. RESULTS: For the in vitro study, chondrocyte proliferation in 5.0 mg/ml PG-atelocollagen gel was enhanced, and the gene expression of Col2a1 and Aggrecan were decreased. In contrast, chondrocyte proliferation in 0.1 and 1.0 mg/ml PG-atelocollagen gel was not enhanced. The gene expression of Aggrecan in 0.1 and 1.0 mg/ml PG-atelocollagen gel was increased. For the in vivo study, the histological average total score of the 0.1 mg/ml PG-atelocollagen gel was significantly better than that of the group without PG. CONCLUSIONS: Although the appropriate concentration of PG has not been defined, this study suggests the efficacy of PG for cartilage repair.

SUBMITTER: Ohshika S 

PROVIDER: S-EPMC3311805 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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Potential of exogenous cartilage proteoglycan as a new material for cartilage regeneration.

Ohshika Shusa S   Ishibashi Yasuyuki Y   Kon Atsushi A   Kusumi Tomomi T   Kijima Hiroshi H   Toh Satoshi S  

International orthopaedics 20110812 4


<h4>Background</h4>Although proteoglycan (PG) is one of the major components of cartilage matrices, its biological function is not fully elucidated.<h4>Methods</h4>The objectives of this study were to investigate the proliferation and differentiation of chondrocytes embedded in atelocollagen gel with exogenous cartilage PG (PG-atelocollagen gel) in vitro, and also to evaluate the repair of cartilage defects by PG-atelocollagen gel in vivo. In the in vitro study, rabbit chondrocytes were cultured  ...[more]

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