Root anatomy and element distribution vary between two Salix caprea isolates with different Cd accumulation capacities.
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ABSTRACT:
SUBMITTER: Vaculik M
PROVIDER: S-EPMC3314946 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:A Gram-reaction-negative, yellow-pigmented strain, designated EX36(T), was characterized using a polyphasic approach comprising phylogenetic, morphological and genotypic analyses. The endophytic strain was isolated from Zn/Cd-accumulating Salix caprea in Arnoldstein, Austria. Analysis of the 16S rRNA gene demonstrated that the novel strain is most closely related to members of the genus Spirosoma (95 % sequence similarity with Spirosoma linguale). The genomic DNA G+C content was 47.2 mol%. The predominant quinone was and the major cellular fatty acids were summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c), C16 : 1ω5c, iso-C17 : 0 3-OH and iso-C15 : 0. On the basis of its phenotypic and genotypic properties, strain EX36(T) should be classified as a novel species of the genus Spirosoma, for which the name Spirosoma endophyticum sp. nov. is proposed. The type strain is EX36(T) ( = DSM 26130(T) = LMG 27272(T)).
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Project description:Salix caprea is well suited for phytoextraction strategies. In a previous survey we showed that genetically distinct S. caprea plants isolated from metal-polluted and unpolluted sites differed in their zinc (Zn) and cadmium (Cd) tolerance and accumulation abilities. To determine the molecular basis of this difference we examined putative homologues of genes involved in heavy metal responses and identified over 200 new candidates with a suppression subtractive hybridization (SSH) screen. Quantitative expression analyses of 20 genes in leaves revealed that some metallothioneins and cell wall modifying genes were induced irrespective of the genotype's origin and metal uptake capacity while a cysteine biosynthesis gene was expressed constitutively higher in the metallicolous genotype. The third and largest group of genes was only induced in the metallicolous genotype. These data demonstrate that naturally adapted woody non-model species can help to discover potential novel molecular mechanisms for metal accumulation and tolerance.
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Project description:In dioecious, zoophilous plants potential pollinators have to be attracted to both sexes and switch between individuals of both sexes for pollination to occur. It often has been suggested that males and females require different numbers of visits for maximum reproductive success because male fertility is more likely limited by access to mates, whereas female fertility is rather limited by resource availability. According to sexual selection theory, males therefore should invest more in pollinator attraction (advertisement, reward) than females. However, our knowledge on the sex specific investment in floral rewards and advertisement, and its effects on pollinator behaviour is limited. Here, we use an approach that includes chemical, spectrophotometric, and behavioural studies i) to elucidate differences in floral nectar reward and advertisement (visual, olfactory cues) in dioecious sallow, Salix caprea, ii) to determine the relative importance of visual and olfactory floral cues in attracting honey bee pollinators, and iii) to test for differential attractiveness of female and male inflorescence cues to honey bees. Nectar amount and sugar concentration are comparable, but sugar composition varies between the sexes. Olfactory sallow cues are more attractive to honey bees than visual cues; however, a combination of both cues elicits the strongest behavioural responses in bees. Male flowers are due to the yellow pollen more colourful and emit a higher amount of scent than females. Honey bees prefer the visual but not the olfactory display of males over those of females. In all, the data of our multifaceted study are consistent with the sexual selection theory and provide novel insights on how the model organism honey bee uses visual and olfactory floral cues for locating host plants.
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Project description:Salix matsudana Koidz. is a fast growing tree species. It has a high cadmium (Cd) tolerance capacity, making it potentially suitable for phytoremediation. Presently, transcriptomic and physiological Cd response mechanisms are poorly understood. Transcriptomic analysis in early response to high (50 ?M) Cd levels was investigated in leaf and root of Cd-resistant S. matsudana Koidz..Analysis of the response profiles demonstrate the existence of a complex transcriptional network in the root and leaf when exposed to Cd. The main response in the root involved up-regulation of genes associated with defence response via callose deposition in the cell wall and cell wall thickening. In the leaf, transcripts related to biotic stress signalling and secondary metabolism were activated. Additionally, many lignin and brassinosteroids synthesis pathway genes were induced mainly in the leaf, indicating that gene response to Cd was tissue-specific. The Cd transcriptome results were consistent with observed physiological changes.The sub-localization, transcriptional network, and physiological regulation demonstrate the tissue-specific manner of Cd response, and provide a novel insight into in early response of tree species to Cd exposure.
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Project description:The Salicaceae family comprises a large number of high-biomass species with remarkable genetic variability and adaptation to ecological niches. Salix caprea survives in heavy metal contaminated areas, translocates and accumulates Zn/Cd in leaves. To reveal potential selective effects of long-term heavy metal contaminations on the genetic structure and Zn/Cd accumulation capacity, 170 S. caprea isolates of four metal-contaminated and three non-contaminated middle European sites were analysed with microsatellite markers using Wright's F statistics. The differentiation of populations North of the Alps are more pronounced compared to the Southern ones. By grouping the isolates based on their contamination status, a weak but significant differentiation was calculated between Northern metallicolous and non-metallicolous populations. To quantify if the contamination and genetic status of the populations correlate with Zn/Cd tolerance and the accumulation capacity, the S. caprea isolates were exposed to elevated Cd/Zn concentrations in perlite-based cultures. Consistent with the genetic data nested anova analyses for the physiological traits find a significant difference in the Cd accumulation capacity between the Northern and Southern populations. Our data suggest that natural populations are a profitable source to uncover genetic mechanisms of heavy metal accumulation and biomass production, traits that are essential for improving phytoextraction strategies.
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Project description:BACKGROUND:Root-to-shoot translocation of zinc (Zn) and cadmium (Cd) depends on the concentrations of both metals in the medium. A previous study on tobacco (Nicotiana tabacum) pointed to the contribution of NtZIP1, NtZIP2, NtZIP4 and NtIRT1-like in the regulation of this phenomenon. To learn more, Zn and Cd accumulation, root/shoot distribution and the expression of ZIP genes were investigated in the apical, middle and basal root parts. RESULTS:We show that Zn/Cd status-dependent root-shoot distribution of both metals was related to distinct metal accumulation in root parts. At low Zn and Cd in the medium, the apical part contained the highest metal level; at higher concentrations, the middle and basal parts were the major sink for excess metal. The above were accompanied by root part-specific expression pattern modifications of ZIPs (NtZIP1-like, NtZIP2, NtZIP4A/B, NtZIP5A/B, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like) that fell into four categories with respect to the root part. Furthermore, for lower Zn/Cd concentrations changes were noted for NtZIP5A/B and NtZIP5-like only, but at higher Zn and Cd levels for NtZIP1-like, NtZIP5-like, NtZIP8, NtZIP11, NtIRT1, and NtIRT1-like. NtZIP1, here renamed to NtZIP5B, was cloned and characterized. We found that it was a zinc deficiency-inducible transporter involved in zinc and cadmium uptake from the soil solution primarily by the middle root part. CONCLUSIONS:We conclude that regulation of the longitudinal distribution of Zn and Cd is highly specific, and that the apical, middle and basal root parts play distinct roles in Zn/Cd status-dependent control of metal translocation efficiency to shoots, including the stimulation of Zn translocation to shoots in the presence of Cd. These results provide new insight into the root part-specific unique role of NtZIP5B and other ZIP genes in the longitudinal distribution of zinc and cadmium and their contribution to the regulation of root-to-shoot translocation.
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Project description:Population and land management relies on understanding population regulation and growth, which may be impacted by variation in population growth parameters within and among populations. We explored the interactions between variation in carrying capacity (K), intrinsic population growth rate (r), and strength of density dependence (β) within and among elk (Cervus elaphus) herds in a small part of the geographic range of the species. We also estimated stochastic fluctuations in abundance around K for each herd. We fit linear Ricker growth models using Bayesian statistics to seven time series of elk population survey data. Our results indicate that K and β varied among herds, and that r and β varied temporally within herds. We also found that herds with smaller K had less stochastic fluctuation in abundances around K, but higher temporal variation in β within herds. Population regulation and the rate of return to the equilibrium abundance is often understood in terms of β, but ecological populations are dynamic systems, and temporal variation in population growth parameters may also influence regulation. Population models which accommodate variation both within and among herds in population growth parameters are necessary, even in mild climates, to fully understand population dynamics and manage populations.
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Project description:Salix integra is a shrub willow native to northeastern China, Japan, Korea, and Primorsky Krai in the far southeast of Russia, and has been identified as cadmium (Cd)-accumulating trees in recent years. Although many physiological studies have been conducted with these plants, little is known about the molecular basis underlying Cd response in this plant, and this is confirmed by the very few number of gene sequences (only 39 nucleotide sequences) available in public databases. Advances in genomics for Salix are promising for future improvement in identification of new candidate genes involved in metal tolerance and accumulation. Thus, high-throughput transcriptome sequencing is essential for generating enormous transcript sequences from S. integra, especially for the purpose of Cd toxicity-responsive genes discovery. Using Illumina paired-end sequencing, approximately 60.05 million high-quality reads were obtained. De novo assembly yielded 80,105 unigenes with an average length of 703 bp, A total of 50,221 (63%) unigenes were further functionally annotated by comparing their sequences to different proteins and functional domain databases. GO annotation reveals 1849 Cd responsive genes involving in Cd binding, transport, and detoxification and cellular Cd homeostasis, and these genes were highly enriched in plant response to Cd ion and Cd ion transport. By searching against the PlantCyc database, 509 unigenes were assigned to 14 PlantCyc pathways related to Cd transport and cellular detoxification, and many of them are genes encoding heavy metal ATPases (HMAs), nature resistance-associated with microphage proteins (NRAMPs), ATP-binding cassette (ABC) transporters, etc., Comprehensive RT-qPCR analysis of these selected genes in different tissues of S. integra under the control and Cd treatment revealed metallothionein-like protein (MT2A and MT2B), Metal tolerance protein (MTP1), ABCB25, NRAMP5, and ZIP1 may be involved in the Cd transport and detoxification in leaves, while NRAMP2, ZIP8, and NRAMP5 may be related to Cd transport in roots. Our study will enrich the sequence information of S. integra in public database, and would provide some new understanding of the molecular mechanisms of heavy metal tolerance and detoxification in willows.
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Project description:Microplastics (MPs) occur widely in terrestrial ecosystems. However, information on the interaction of MPs with metals in terrestrial ecosystems is lacking in the literature. The present study investigated the effects of two types of MPs (high-density polyethylene (HDPE) and polystyrene (PS)) with different dosages (i.e., 0, 0.1%, 1%, and 10%) on the uptake and effects of Cd in maize plants grown in an agricultural soil. Results showed that addition of Cd at a 5 mg/kg caused inhibited plant growth and resulted in high Cd accumulation in plant tissues. Polyethylene alone showed no significant phytotoxic effects, but a high-dose of HDPE (10%) amplified Cd phytotoxicity. Polystyrene negatively affected maize growth and phytoxicity further increased in the presence of Cd. Both HDPE and PS caused soil diethylenetriaminepentaacetic acid (DTPA)-extractable Cd concentrations to increase but did not significantly affect Cd uptake into plant tissues. In the soil without Cd addition, HDPE decreased soil pH, while PS did not significantly alter soil pH. However, in the soil spiked with Cd, both HDPE and PS increased pH. Overall, impacts on plant growth and Cd accumulation varied with MP type and dose, and PS induced substantial phytotoxicity. In conclusion, co-occurring MPs can change Cd bioavailability, plant performance, and soil traits. Our findings highlight the ecological impacts that could occur from the release of MPs into soil.
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