Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment.
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ABSTRACT:
SUBMITTER: McConnell MJ
PROVIDER: S-EPMC3318517 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment.
McConnell Michael J MJ Pérez-Ordóñez Ana A Pérez-Romero Pilar P Valencia Raquel R Lepe José Antonio JA Vázquez-Barba Isabel I Pachón Jerónimo J
Journal of clinical microbiology 20120201 4
A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA tha ...[more]
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