The molecular architecture of human Dicer.
Ontology highlight
ABSTRACT:
SUBMITTER: Lau PW
PROVIDER: S-EPMC3319852 | biostudies-literature | 2012 Mar
REPOSITORIES: biostudies-literature
Publications
The molecular architecture of human Dicer.
Lau Pick-Wei PW Guiley Keelan Z KZ De Nabanita N Potter Clinton S CS Carragher Bridget B MacRae Ian J IJ
Nature structural & molecular biology 20120318 4
Dicer is a multidomain enzyme that generates small RNAs for gene silencing in eukaryotes. Current understanding of Dicer structure is restricted to simple forms of the enzyme, whereas that of the large and complex Dicer in metazoans is unknown. Here we describe a new domain localization strategy developed to determine the structure of human Dicer by EM. A rearrangement of the nuclease core, compared to the archetypal Giardia lamblia Dicer, explains how metazoan Dicers generate products that are ...[more]
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