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DeSUMOylating isopeptidase: a second class of SUMO protease.

ABSTRACT: The modification of proteins by small ubiquitin-like modifier (SUMO) is crucial for the regulation of diverse cellular processes. Protein SUMOylation is reversed by isopeptidases, collectively known as deSUMOylases. Only one family of SUMO-specific proteases has been described so far: the sentrin-specific proteases (SENP). Here, we identify and characterize a new deSUMOylase, which we have named DeSI-1 (DeSumoylating Isopeptidase 1). We describe BZEL—a new transcriptional repressor—as substrate of DeSI-1. DeSI-1 catalyses the deSUMOylation, but not the deubiquitination, of BZEL. Furthermore, the SENP substrates PML and ?Np63 are not deSUMOylated by DeSI-1, suggesting that SENP and DeSI enzymes recognize different sets of substrates. Together, these data identify a second class of SUMO proteases.

SUBMITTER: Shin EJ

PROVIDER: S-EPMC3321169 | biostudies-literature | 2012 Apr

REPOSITORIES: biostudies-literature

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DeSUMOylating isopeptidase: a second class of SUMO protease.

Shin Eun Ju EJ Shin Hyun Mi HM Nam Eori E Kim Won Seog WS Kim Ji-Hoon JH Oh Byung-Ha BH Yun Yungdae Y

EMBO reports 20120401 4

The modification of proteins by small ubiquitin-like modifier (SUMO) is crucial for the regulation of diverse cellular processes. Protein SUMOylation is reversed by isopeptidases, collectively known as deSUMOylases. Only one family of SUMO-specific proteases has been described so far: the sentrin-specific proteases (SENP). Here, we identify and characterize a new deSUMOylase, which we have named DeSI-1 (DeSumoylating Isopeptidase 1). We describe BZEL—a new transcriptional repressor—as substrate ...[more]

PMID: 22370726

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