Unknown

Dataset Information

0

The SUMO-specific isopeptidase SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic ?-helix.


ABSTRACT: Sumoylation regulates a wide range of essential cellular functions, many of which are associated with activities in the nucleus. Although there is also emerging evidence for the involvement of the small ubiquitin-related modifier (SUMO) at intracellular membranes, the mechanisms by which sumoylation is regulated at membranes is largely unexplored. In this study, we report that the SUMO-specific isopeptidase, SENP2, uniquely associates with intracellular membranes. Using in vivo analyses and in vitro binding assays, we show that SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic ?-helix that promotes direct membrane binding. Furthermore, we demonstrate that SENP2 binding to intracellular membranes is regulated by interactions with the nuclear import receptor karyopherin-?. Consistent with membrane association, biotin identification (BioID) revealed interactions between SENP2 and endoplasmic reticulum, Golgi, and inner nuclear membrane-associated proteins. Collectively, our findings indicate that SENP2 binds to intracellular membranes where it interacts with membrane-associated proteins and has the potential to regulate their sumoylation and membrane-associated functions.

SUBMITTER: Odeh HM 

PROVIDER: S-EPMC6085828 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

The SUMO-specific isopeptidase SENP2 is targeted to intracellular membranes via a predicted N-terminal amphipathic α-helix.

Odeh Hana M HM   Coyaud Etienne E   Raught Brian B   Matunis Michael J MJ  

Molecular biology of the cell 20180606 15


Sumoylation regulates a wide range of essential cellular functions, many of which are associated with activities in the nucleus. Although there is also emerging evidence for the involvement of the small ubiquitin-related modifier (SUMO) at intracellular membranes, the mechanisms by which sumoylation is regulated at membranes is largely unexplored. In this study, we report that the SUMO-specific isopeptidase, SENP2, uniquely associates with intracellular membranes. Using in vivo analyses and in v  ...[more]

Similar Datasets

| S-EPMC8857072 | biostudies-literature
| S-EPMC3237629 | biostudies-literature
| S-EPMC5435892 | biostudies-literature
| S-EPMC3321169 | biostudies-literature
| S-EPMC241219 | biostudies-other
| S-EPMC1220354 | biostudies-other
| S-EPMC3020963 | biostudies-literature
| S-EPMC7760104 | biostudies-literature
| S-EPMC2778571 | biostudies-literature
| S-EPMC4505600 | biostudies-literature