Ontology highlight
ABSTRACT:
SUBMITTER: Yogo K
PROVIDER: S-EPMC3322154 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Yogo Katsunori K Ogawa Taisaku T Hayashi Masahito M Harada Yoshie Y Nishizaka Takayuki T Kinosita Kazuhiko K
PloS one 20120409 4
Type-II DNA topoisomerases resolve DNA entanglements such as supercoils, knots and catenanes by passing one segment of DNA duplex through a transient enzyme-bridged double-stranded break in another segment. The ATP-dependent passage reaction has previously been demonstrated at the single-molecule level, showing apparent processivity at saturating ATP. Here we directly observed the strand passage by human topoisomerase IIα, after winding a pair of fluorescently stained DNA molecules with optical ...[more]