Ontology highlight
ABSTRACT:
SUBMITTER: Martinez-Garcia B
PROVIDER: S-EPMC3919613 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Martínez-García Belén B Fernández Xavier X Díaz-Ingelmo Ofelia O Rodríguez-Campos Antonio A Manichanh Chaysavanh C Roca Joaquim J
Nucleic acids research 20131031 3
By transporting one DNA double helix (T-segment) through a double-strand break in another (G-segment), topoisomerase II reduces fractions of DNA catenanes, knots and supercoils to below equilibrium values. How DNA segments are selected to simplify the equilibrium DNA topology is enigmatic, and the biological relevance of this activity is unclear. Here we examined the transit of the T-segment across the three gates of topoisomerase II (entry N-gate, DNA-gate and exit C-gate). Our experimental res ...[more]