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Peptide nucleic acids as tools for single-molecule sequence detection and manipulation.

ABSTRACT: The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to individual double-stranded (ds) DNA molecules is critical to a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose and implement them in two model single-molecule experiments where individual DNA molecules are manipulated via microfluidic flow and optical tweezers, respectively. We demonstrate that PNAs are versatile and robust sequence-specific tethers.

SUBMITTER: Zohar H

PROVIDER: S-EPMC3322611 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Peptide nucleic acids as tools for single-molecule sequence detection and manipulation.

Zohar Hagar H Hetherington Craig L CL Bustamante Carlos C Muller Susan J SJ

Nano letters 20101101 11

The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to individual double-stranded (ds) DNA molecules is critical to a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose and implement them in two model single-molecule experiments where individual DNA molecules are manipulated via microfluidic flow and optical tweezers, respectively. We demonstrate that PNAs are versatile and robust seq ...[more]

PMID: 20923183

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