Immunofluorescence assay for serologic diagnosis of SARS.
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ABSTRACT:
SUBMITTER: Chan PK
PROVIDER: S-EPMC3322771 | biostudies-literature | 2004 Mar
REPOSITORIES: biostudies-literature
Publications
Immunofluorescence assay for serologic diagnosis of SARS.
Chan Paul K S PK Ng King-Cheung KC Chan Rickjason C W RC Lam Rebecca K Y RK Chow Viola C Y VC Hui Mamie M Wu Alan A Lee Nelson N Yap Florence H Y FH Cheng Frankie W T FW Sung Joseph J Y JJ Tam John S JS
Emerging infectious diseases 20040301 3
We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection. ...[more]
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