PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.
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ABSTRACT:
SUBMITTER: Gale N
PROVIDER: S-EPMC3324338 | biostudies-literature | 2011 Jul-Dec
REPOSITORIES: biostudies-literature
Publications
PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.
Gale Nittaya N Kocalka Petr P Mardle Charlotte C Brown Tom T
Artificial DNA, PNA & XNA 20110701 3
Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting. ...[more]
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