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Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy.


ABSTRACT: A combination of targeted probes and new imaging technologies provides a powerful set of tools with the potential to improve the early detection of cancer. To develop a probe for detecting colon cancer, we screened phage display peptide libraries against fresh human colonic adenomas for high-affinity ligands with preferential binding to premalignant tissue. We identified a specific heptapeptide sequence, VRPMPLQ, which we synthesized, conjugated with fluorescein and tested in patients undergoing colonoscopy. We imaged topically administered peptide using a fluorescence confocal microendoscope delivered through the instrument channel of a standard colonoscope. In vivo images were acquired at 12 frames per second with 50-microm working distance and 2.5-microm (transverse) and 20-microm (axial) resolution. The fluorescein-conjugated peptide bound more strongly to dysplastic colonocytes than to adjacent normal cells with 81% sensitivity and 82% specificity. This methodology represents a promising diagnostic imaging approach for the early detection of colorectal cancer and potentially of other epithelial malignancies.

SUBMITTER: Hsiung PL 

PROVIDER: S-EPMC3324975 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Detection of colonic dysplasia in vivo using a targeted heptapeptide and confocal microendoscopy.

Hsiung Pei-Lin PL   Hardy Jonathan J   Friedland Shai S   Soetikno Roy R   Du Christine B CB   Wu Amy P AP   Sahbaie Peyman P   Crawford James M JM   Lowe Anson W AW   Contag Christopher H CH   Wang Thomas D TD  

Nature medicine 20080316 4


A combination of targeted probes and new imaging technologies provides a powerful set of tools with the potential to improve the early detection of cancer. To develop a probe for detecting colon cancer, we screened phage display peptide libraries against fresh human colonic adenomas for high-affinity ligands with preferential binding to premalignant tissue. We identified a specific heptapeptide sequence, VRPMPLQ, which we synthesized, conjugated with fluorescein and tested in patients undergoing  ...[more]

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