Ontology highlight
ABSTRACT:
SUBMITTER: Hasan NM
PROVIDER: S-EPMC3325154 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Hasan N M NM Longacre M J MJ Stoker S W SW Kendrick M A MA Druckenbrod N R NR Laychock S G SG Mastrandrea L D LD MacDonald M J MJ
Archives of biochemistry and biophysics 20111204 1
To evaluate the role of sphingosine kinase 1 (SphK1) in insulin secretion, we used stable transfection to knock down the expression of the Sphk1 gene in the rat insulinoma INS-1 832/13 cell line. Cell lines with lowered Sphk1 mRNA expression and SphK1 enzyme activity (SK11 and SK14) exhibited lowered glucose- and 2-aminobicyclo[2,2,1]heptane-2-carboxylic acid (BCH) plus glutamine-stimulated insulin release and low insulin content associated with decreases in the mRNA of the insulin 1 gene. Overe ...[more]