Towards the crystal structure elucidation of eukaryotic UDP-galactopyranose mutase.
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ABSTRACT:
SUBMITTER: van Straaten KE
PROVIDER: S-EPMC3325819 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Towards the crystal structure elucidation of eukaryotic UDP-galactopyranose mutase.
van Straaten Karin E KE Routier Francoise H FH Sanders David A R DA
Acta crystallographica. Section F, Structural biology and crystallization communications 20120328 Pt 4
UDP-galactopyranose mutase (UGM) catalyzes the interconversion of UDP-galactopyranose and UDP-galactofuranose. Eukaryotic UGMs from Aspergillus fumigatus and Leishmania major have been purified to homogeneity by means of Ni(2+)-affinity chromatography and crystallized. Eukaryotic UGM structure elucidation was not straightforward owing to high pseudo-symmetry, twinning and very low anomalous signal. Phasing to 2.8 Å resolution using SAD was successful for L. major UGM. However, the maps could onl ...[more]
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