Ontology highlight
ABSTRACT:
SUBMITTER: Agelopoulos M
PROVIDER: S-EPMC3329935 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Agelopoulos Marios M McKay Daniel J DJ Mann Richard S RS
Cell reports 20120401 4
We present a strategy to examine the chromatin conformation of individual loci in specific cell types during Drosophila embryogenesis. Regulatory DNA is tagged with binding sites (lacO) for LacI, which is used to immunoprecipitate the tagged chromatin from specific cell types. We applied this approach to Distalless (Dll), a gene required for limb development in Drosophila. We show that the local chromatin conformation at Dll depends on the cell type: in cells that express Dll, the 5' regulatory ...[more]