Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.
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ABSTRACT:
SUBMITTER: Lenn T
PROVIDER: S-EPMC3337751 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Publications
Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.
Lenn Tchern T Gkekas Christos N CN Bernard Laurent L Engl Christoph C Jovanovic Goran G Buck Martin M Ying Liming L
Chemical communications (Cambridge, England) 20100907 1
We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells. ...[more]
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