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Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.

ABSTRACT: We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.

SUBMITTER: Lenn T 

PROVIDER: S-EPMC3337751 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Measuring the stoichiometry of functional PspA complexes in living bacterial cells by single molecule photobleaching.

Lenn Tchern T   Gkekas Christos N CN   Bernard Laurent L   Engl Christoph C   Jovanovic Goran G   Buck Martin M   Ying Liming L  

Chemical communications (Cambridge, England) 20100907 1

We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells. ...[more]

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