Ontology highlight
ABSTRACT:
SUBMITTER: Steichen JM
PROVIDER: S-EPMC3340281 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Steichen Jon M JM Kuchinskas Michael M Keshwani Malik M MM Yang Jie J Adams Joseph A JA Taylor Susan S SS
The Journal of biological chemistry 20120210 18
The catalytic subunit of cAMP-dependent protein kinase (PKA) is a member of the AGC group of protein kinases. Whereas PKA has served as a structural model for the protein kinase superfamily, all previous structures of the catalytic subunit contain a phosphorylated activation loop. To understand the structural effects of activation loop phosphorylation at Thr-197 we used a PKA mutant that does not autophosphorylate at Thr-197. The enzyme crystallized in the apo-state, and the structure was solved ...[more]