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Unexpected high digestion rate of cooked starch by the Ct-maltase-glucoamylase small intestine mucosal ?-glucosidase subunit.

ABSTRACT: For starch digestion to glucose, two luminal ?-amylases and four gut mucosal ?-glucosidase subunits are employed. The aim of this research was to investigate, for the first time, direct digestion capability of individual mucosal ?-glucosidases on cooked (gelatinized) starch. Gelatinized normal maize starch was digested with N- and C-terminal subunits of recombinant mammalian maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) of varying amounts and digestion periods. Without the aid of ?-amylase, Ct-MGAM demonstrated an unexpected rapid and high digestion degree near 80%, while other subunits showed 20 to 30% digestion. These findings suggest that Ct-MGAM assists ?-amylase in digesting starch molecules and potentially may compensate for developmental or pathological amylase deficiencies.

SUBMITTER: Lin AH 

PROVIDER: S-EPMC3341394 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Unexpected high digestion rate of cooked starch by the Ct-maltase-glucoamylase small intestine mucosal α-glucosidase subunit.

Lin Amy Hui-Mei AH   Nichols Buford L BL   Quezada-Calvillo Roberto R   Avery Stephen E SE   Sim Lyann L   Rose David R DR   Naim Hassan Y HY   Hamaker Bruce R BR  

PloS one 20120501 5

For starch digestion to glucose, two luminal α-amylases and four gut mucosal α-glucosidase subunits are employed. The aim of this research was to investigate, for the first time, direct digestion capability of individual mucosal α-glucosidases on cooked (gelatinized) starch. Gelatinized normal maize starch was digested with N- and C-terminal subunits of recombinant mammalian maltase-glucoamylase (MGAM) and sucrase-isomaltase (SI) of varying amounts and digestion periods. Without the aid of α-amy  ...[more]

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