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Supramolecular displacement-mediated activation of a silent fluorescence probe for label-free ligand screening.

ABSTRACT: We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe from the protein's binding pocket, a fluorescence profile is generated. This profile is used to discriminate analytes based on their relative binding affinities.

SUBMITTER: Torres DA

PROVIDER: S-EPMC3342419 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Supramolecular displacement-mediated activation of a silent fluorescence probe for label-free ligand screening.

Torres Diego Amado DA Azagarsamy Malar A MA Thayumanavan S S

Journal of the American Chemical Society 20120423 17

We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe from the protein's binding pocket, a fluorescence profile is generated. This profile is used to discriminate analytes based on their relative binding affinities. ...[more]

PMID: 22500607

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