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Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique.


ABSTRACT: We screened a randomized library and identified natural peptides that bound selectively to a chimeric peptide containing ?-, ?- and ?-amino acids. The SPOT arrays provide a means for the systematic study of the possible interaction space accessible to the ???-chimera. The mutational analysis reveals the dependence of the binding affinities of ?-peptides to the ???-chimera, on the hydrophobicity and bulkiness of the side chains at the corresponding hydrophobic interface. The stability of the resulting heteroassemblies was further confirmed in solution by CD and thermal denaturation.

SUBMITTER: Rezaei Araghi R 

PROVIDER: S-EPMC3343290 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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