Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers.
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ABSTRACT:
SUBMITTER: Tymensen LD
PROVIDER: S-EPMC3346394 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Publications
Community structure analysis of methanogens associated with rumen protozoa reveals bias in universal archaeal primers.
Tymensen Lisa D LD McAllister Tim A TA
Applied and environmental microbiology 20120323 11
The diversity of protozoan-associated methanogens in cattle was investigated using five universal archaeal small-subunit (SSU) rRNA gene primer sets. Methanobrevibacter spp. and rumen cluster C (distantly related to Thermoplasma spp.) were predominant. Significant differences in species composition among libraries indicate that some primers used previously to characterize rumen methanogens exhibit biased amplification. ...[more]
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