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Establishment of a tractable genetic transformation system in Veillonella spp.


ABSTRACT: We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies.

SUBMITTER: Liu J 

PROVIDER: S-EPMC3346448 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Establishment of a tractable genetic transformation system in Veillonella spp.

Liu Jinman J   Xie Zhoujie Z   Merritt Justin J   Qi Fengxia F  

Applied and environmental microbiology 20120217 9


We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies. ...[more]

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