Scaling down the analysis of environmental processes: monitoring enzyme activity in natural substrates on a millimeter resolution scale.
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ABSTRACT:
SUBMITTER: Baldrian P
PROVIDER: S-EPMC3346463 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
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Scaling down the analysis of environmental processes: monitoring enzyme activity in natural substrates on a millimeter resolution scale.
Baldrian Petr P Vetrovsky Tomás T
Applied and environmental microbiology 20120302 9
Natural environments often show high levels of spatial heterogeneity. With a methodology based on the immobilization of fluorescent substrates, the distribution of extracellular enzymes can be studied at a 2.3-mm resolution with a detection limit of 1.8 nmol · h(-1) · cm(-2). The method is applicable to environmental samples such as wood, litter, soil, or fungal colonies. ...[more]
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