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Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry.

ABSTRACT: Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococcus aureus. The directionally released antibiotic alters S. aureus virulence factor production and colonization. Therefore, IMS provides insight into the largely hidden nature of competitive microbial encounters and niche establishment, and provides a paradigm for future antibiotic discovery.

SUBMITTER: Gonzalez DJ

PROVIDER: S-EPMC3352172 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature

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Microbial competition between Bacillus subtilis and Staphylococcus aureus monitored by imaging mass spectrometry.

Gonzalez David J DJ Haste Nina M NM Hollands Andrew A Fleming Tinya C TC Hamby Matthew M Pogliano Kit K Nizet Victor V Dorrestein Pieter C PC

Microbiology (Reading, England) 20110630 Pt 9

Microbial competition exists in the general environment, such as soil or aquatic habitats, upon or within unicellular or multicellular eukaryotic life forms. The molecular actions that govern microbial competition, leading to niche establishment and microbial monopolization, remain undetermined. The emerging technology of imaging mass spectrometry (IMS) enabled the observation that there is directionality in the metabolic output of the organism Bacillus subtilis when co-cultured with Staphylococ ...[more]

PMID: 21719540

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