Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.
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ABSTRACT:
SUBMITTER: Upton H
PROVIDER: S-EPMC3668096 | biostudies-literature | 2012 Apr
REPOSITORIES: biostudies-literature
Publications
Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.
Upton Heather H Newton Gerald L GL Gushiken Melissa M Lo Kelly K Holden Dhiraj D Fahey Robert C RC Rawat Mamta M
FEBS letters 20120306 7
The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol bi ...[more]
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