Development of a universal glycosyltransferase assay amenable to high-throughput formats.
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ABSTRACT:
SUBMITTER: Lee HS
PROVIDER: S-EPMC3354722 | biostudies-literature | 2011 Nov
REPOSITORIES: biostudies-literature
Publications
Development of a universal glycosyltransferase assay amenable to high-throughput formats.
Lee Hyun Soo HS Thorson Jon S JS
Analytical biochemistry 20110708 1
The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization. ...[more]
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