Atomic force mechanobiology of pluripotent stem cell-derived cardiomyocytes.
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ABSTRACT:
SUBMITTER: Liu J
PROVIDER: S-EPMC3356329 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Atomic force mechanobiology of pluripotent stem cell-derived cardiomyocytes.
Liu Jianwei J Sun Ning N Bruce Marc A MA Wu Joseph C JC Butte Manish J MJ
PloS one 20120518 5
We describe a method using atomic force microscopy (AFM) to quantify the mechanobiological properties of pluripotent, stem cell-derived cardiomyocytes, including contraction force, rate, duration, and cellular elasticity. We measured beats from cardiomyocytes derived from induced pluripotent stem cells of healthy subjects and those with dilated cardiomyopathy, and from embryonic stem cell lines. We found that our AFM method could quantitate beat forces of single cells and clusters of cardiomyocy ...[more]
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