A metagenome of a full-scale microbial community carrying out enhanced biological phosphorus removal.

ABSTRACT: Enhanced biological phosphorus removal (EBPR) is widely used for removal of phosphorus from wastewater. In this study, a metagenome (18.2 Gb) was generated using Illumina sequencing from a full-scale EBPR plant to study the community structure and genetic potential. Quantitative fluorescence in situ hybridization (qFISH) was applied as an independent method to evaluate the community structure. The results were in qualitative agreement, but a DNA extraction bias against gram positive bacteria using standard extraction protocols was identified, which would not have been identified without the use of qFISH. The genetic potential for community function showed enrichment of genes involved in phosphate metabolism and biofilm formation, reflecting the selective pressure of the EBPR process. Most

SUBMITTER: Albertsen M 

PROVIDER: S-EPMC3358022 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature