Ontology highlight
ABSTRACT:
SUBMITTER: Lu MS
PROVIDER: S-EPMC3358930 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Lu Michelle S MS Mauser Jonathon F JF Prehoda Kenneth E KE
ACS synthetic biology 20120201 2
Cellular protein interaction networks exhibit sigmoidal input-output relationships with thresholds and steep responses (i.e., ultrasensitivity). Although cooperativity can be a source of ultrasensitivity, we examined whether the presence of "decoy" binding sites that are not coupled to activation could also lead to this effect. To systematically vary key parameters of the system, we designed a synthetic regulatory system consisting of an autoinhibited PDZ domain coupled to an activating SH3 doma ...[more]