Dataset Information

Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica.

ABSTRACT: To advance the understanding of the molecular mechanisms controlling microbial activities involved in carbon cycling and mitigation of environmental pollution in freshwaters, the influence of heavy metals and natural as well as xenobiotic organic compounds on laccase gene expression was quantified using quantitative real-time PCR (qRT-PCR) in an exclusively aquatic fungus (the aquatic hyphomycete Clavariopsis aquatica) for the first time. Five putative laccase genes (lcc1 to lcc5) identified in C. aquatica were differentially expressed in response to the fungal growth stage and potential laccase inducers, with certain genes being upregulated by, e.g., the lignocellulose breakdown product vanillic acid, the endocrine disruptor technical nonylphenol, manganese, and zinc. lcc4 is inducible by vanillic acid and most likely encodes an extracellular laccase already excreted during the trophophase of the organism, suggesting a function during fungal substrate colonization. Surprisingly, unlike many laccases of terrestrial fungi, none of the C. aquatica laccase genes was found to be upregulated by copper. However, copper strongly increases extracellular laccase activity in C. aquatica, possibly due to stabilization of the copper-containing catalytic center of the enzyme. Copper was found to half-saturate laccase activity already at about 1.8 ?M, in favor of a fungal adaptation to low copper concentrations of aquatic habitats.

SUBMITTER: Sole M

PROVIDER: S-EPMC3370489 | biostudies-literature | 2012 Jul

REPOSITORIES: biostudies-literature

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Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica.

Solé Magali M Müller Ines I Pecyna Marek J MJ Fetzer Ingo I Harms Hauke H Schlosser Dietmar D

Applied and environmental microbiology 20120427 13

To advance the understanding of the molecular mechanisms controlling microbial activities involved in carbon cycling and mitigation of environmental pollution in freshwaters, the influence of heavy metals and natural as well as xenobiotic organic compounds on laccase gene expression was quantified using quantitative real-time PCR (qRT-PCR) in an exclusively aquatic fungus (the aquatic hyphomycete Clavariopsis aquatica) for the first time. Five putative laccase genes (lcc1 to lcc5) identified in ...[more]

PMID: 22544244

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Project description:Introduction Artificial turf or synthetic grass releases hazardous substances such as heavy metals, polycyclic aromatic hydrocarbons (PAHs), and volatile organic compounds (VOCs). Objective The current study aimed to evaluate the concentration levels of hazardous substances that are emitted from artificial turf as a result of sunlight effect; and to assess the expected exposure risks to such emitted substances during various activities. The current study aimed to evaluate the concentration levels of hazardous substances that are emitted from artificial turf as a result of the effect of sunlight. And to assess the expected risks of exposure to these substances emitted during the various activities. Study design VOCs emitted from artificial turf samples were monitored and collected in the ambient air of three football fields, the ambient air around a piece of new artificial turf that has not yet been used on playing fields, but has been exposed to sunlight within one year and in the indoor air around a piece of new artificial turf. Which has not yet been used on the playgrounds and was placed at room temperature and away from sunlight. Results The current study shows that average afternoon morning VOCs levels were 277, 333, 405 and 509 mg/m3 in winter, autumn, spring and summer, respectively. The most predominant PAHs compounds present in the samples were compounds with 3-rings and 4-rings. The average daily intake (ADI) for three exposure routes (ingestion, inhalation, and dermal contact) was calculated for different age categories (3–6, 7–15, 16–18, 19–22, 23–55, and 56–70 year). Non-Carcinogenic exposure risk as hazard quotient (HQ) and hazard index (HI) were detected. Conclusion All HI values were <1, indicating that there is no potential adverse health effects occur as a result of a chemical exposure. Total carcinogenic risk (R) values for the different age categories were higher than 1E-04 for three football artificial grass fields, which indicated a high cancer risk development probability. HI and R probability increased in the age group of 7–15 year > 3–6 years.

Dataset Information

Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica.

Publications

Differential regulation by organic compounds and heavy metals of multiple laccase genes in the aquatic hyphomycete Clavariopsis aquatica.

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