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Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing.

ABSTRACT: We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ?8.6× high-quality coverage of each strand.

SUBMITTER: Adey A 

PROVIDER: S-EPMC3371708 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Ultra-low-input, tagmentation-based whole-genome bisulfite sequencing.

Adey Andrew A   Shendure Jay J  

Genome research 20120330 6

We have adapted transposase-based in vitro shotgun library construction ("tagmentation") for whole-genome bisulfite sequencing. This method, Tn5mC-seq, enables a >100-fold reduction in starting material relative to conventional protocols, such that we generate highly complex bisulfite sequencing libraries from as little as 10 ng of input DNA, and ample useful sequences from 1 ng of input DNA. We demonstrate Tn5mC-seq by sequencing the methylome of a human lymphoblastoid cell line to ∼8.6× high-q  ...[more]

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