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Improved tagmentation-based whole genome bisulfite sequencing for input DNA from less than 100 cells

ABSTRACT: We improved the tagmentation-based whole genome bisulfite sequencing technology, enabling stable library construction with complexity from minimally 0.5ng of initial genomic DNA, which equals less than 100 cells. This new approach is highly attractive for the complete methylome analysis of very limited cell numbers, e.g., pre-implantation embryos, or tiny biopsy specimens.

ORGANISM(S): Oryza sativa

PROVIDER: GSE57062 | GEO | 2014/05/01

SECONDARY ACCESSION(S): PRJNA245358

REPOSITORIES: GEO

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Improved tagmentation-based whole genome bisulfite sequencing for input DNA from less than 100 cells

Project description:We improved the tagmentation-based whole genome bisulfite sequencing technology, enabling stable library construction with complexity from minimally 0.5ng of initial genomic DNA, which equals less than 100 cells. This new approach is highly attractive for the complete methylome analysis of very limited cell numbers, e.g., pre-implantation embryos, or tiny biopsy specimens. Applying this newly modified T-WGBS, we sequenced the methylome of a rice strain using libraries constructed from 10 ng, 1 ng and 0.5 ng of input genomic DNA. Two libraries were independently constructed and sequenced for each aliquot of input genomic DNA

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