A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine.
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ABSTRACT:
SUBMITTER: Koizumi N
PROVIDER: S-EPMC3372145 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
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A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in urine.
Koizumi Nobuo N Nakajima Chie C Harunari Tsunehito T Tanikawa Tsutomu T Tokiwa Toshihiro T Uchimura Eriko E Furuya Tokujiro T Mingala Claro Niegos CN Villanueva Marvin Ardeza MA Ohnishi Makoto M Suzuki Yasuhiko Y
Journal of clinical microbiology 20120314 6
We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR. ...[more]
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