Ontology highlight
ABSTRACT:
SUBMITTER: Kozlov IA
PROVIDER: S-EPMC3373263 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Kozlov Igor A IA Thomsen Elliot R ER Munchel Sarah E SE Villegas Patricia P Capek Petr P Gower Austin J AJ Pond Stephanie J K SJ Chudin Eugene E Chee Mark S MS
PloS one 20120612 6
We report a scalable and cost-effective technology for generating and screening high-complexity customizable peptide sets. The peptides are made as peptide-cDNA fusions by in vitro transcription/translation from pools of DNA templates generated by microarray-based synthesis. This approach enables large custom sets of peptides to be designed in silico, manufactured cost-effectively in parallel, and assayed efficiently in a multiplexed fashion. The utility of our peptide-cDNA fusion pools was demo ...[more]