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Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.

ABSTRACT: Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were obtained from their interactions with each of a panel of ligands.

SUBMITTER: Kim H

PROVIDER: S-EPMC3375012 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.

Kim Heungbok H Webster Cecelia C Roberts Justin K M JK Kositsawat Juthamas J Hung Li-Wei LW Terwilliger Thomas C TC Kim Chang-Yub CY

Journal of structural and functional genomics 20120601 2

Ligands interacting with Mycobacterium tuberculosis recombinant proteins were identified through use of the ability of Cibacron Blue F3GA dye to interact with nucleoside/nucleotide binding proteins, and the effects of these ligands on crystallization were examined. Co-crystallization with ligands enhanced crystallization and enabled X-ray diffraction data to be collected to a resolution of atleast 2.7 Å for 5 of 10 proteins tested. Additionally, clues about individual proteins’ functions were ob ...[more]

PMID: 22286688

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