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Easy assembly of ligands for glycosidase affinity chromatography.

ABSTRACT: An improved, high-yield synthesis of the corresponding N-carboxypentyl derivatives of three iminoalditol glycosidase inhibitors has been developed for affinity chromatography enzyme purification. Reductive amination of 1-deoxynojirimycin (or its D-manno or D-galacto analogues) with methyl 5-formylvalerate and NaBH3CN at neutral pH afforted an aminoester which upon hydrolysis with aqueous 5% HCl gave the desired aminoacid in 97% overall yield. These amino acids could then be covalently attached using water-soluble carbodi-imide to 6-aminohexyl Sepharose 4B.

SUBMITTER: Bernotas RC

PROVIDER: S-EPMC1131756 | biostudies-other | 1990 Sep

REPOSITORIES: biostudies-other

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Enhancement of crystallization with nucleotide ligands identified by dye-ligand affinity chromatography.

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