Unknown

Dataset Information

0

Determination of neutrophil antigen HNA-3a and HNA-3b genotype frequencies in six racial groups by high-throughput 5' exonuclease assay.


ABSTRACT: People with the human neutrophil antigen (HNA)-3b/3b type can make HNA-3a antibodies, which have been reported to cause immune neutropenia disorders and are especially prone to cause severe cases of transfusion-related acute lung injury. However, knowledge of HNA-3 allele frequencies outside Caucasian populations is limited. We developed a high-throughput genotyping assay and determined the HNA-3a/3b genotype frequencies in six different racial and ethnic groups.Genotyping utilized TaqMan 5' exonuclease chemistry and real-time polymerase chain reaction. A total of 742 DNA samples from six different racial and ethnic groups were genotyped for HNA-3a and HNA-3b.The genotyping assay showed 100% sensitivity and specificity compared to sequencing and phenotyping and had high throughput. A significant percentage of Caucasians (6.5%), Han Chinese (16%), and Asian Indians (6%) typed HNA-3b/3b, but only a small percentage of Hispanics (1%) and no African or Native Americans.The HNA-3 genotyping assay had high sensitivity, specificity, and sample throughput. HNA-3b/b genotype results determined for 742 individuals representing six different racial and ethnic groups showed that there could be a significant risk of producing anti-HNA-3a in Chinese, as well as in Caucasian and Asian Indian blood donor populations, but a very low risk in Hispanic, African, or Native American populations.

SUBMITTER: Bowens KL 

PROVIDER: S-EPMC3376704 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Determination of neutrophil antigen HNA-3a and HNA-3b genotype frequencies in six racial groups by high-throughput 5' exonuclease assay.

Bowens Krista L KL   Sullivan Mia J MJ   Curtis Brian R BR  

Transfusion 20120313 11


<h4>Background</h4>People with the human neutrophil antigen (HNA)-3b/3b type can make HNA-3a antibodies, which have been reported to cause immune neutropenia disorders and are especially prone to cause severe cases of transfusion-related acute lung injury. However, knowledge of HNA-3 allele frequencies outside Caucasian populations is limited. We developed a high-throughput genotyping assay and determined the HNA-3a/3b genotype frequencies in six different racial and ethnic groups.<h4>Study desi  ...[more]

Similar Datasets

| S-EPMC5853077 | biostudies-literature
| S-EPMC2904527 | biostudies-literature
| S-EPMC9133786 | biostudies-literature
| S-EPMC4969071 | biostudies-literature
| S-EPMC2837332 | biostudies-literature
| S-EPMC3849977 | biostudies-literature
2015-03-30 | E-GEOD-63280 | biostudies-arrayexpress
2015-03-30 | GSE63280 | GEO
2012-02-27 | E-GEOD-35200 | biostudies-arrayexpress
| S-EPMC4240753 | biostudies-literature