Ontology highlight
ABSTRACT:
SUBMITTER: Brunger AT
PROVIDER: S-EPMC3380535 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Structure (London, England : 1993) 20120601 6
In X-ray crystallography, molecular replacement and subsequent refinement is challenging at low resolution. We compared refinement methods using synchrotron diffraction data of photosystem I at 7.4 Å resolution, starting from different initial models with increasing deviations from the known high-resolution structure. Standard refinement spoiled the initial models, moving them further away from the true structure and leading to high R(free)-values. In contrast, DEN refinement improved even the m ...[more]