Ontology highlight
ABSTRACT:
SUBMITTER: Moeller A
PROVIDER: S-EPMC3382979 | biostudies-literature | 2012 Feb
REPOSITORIES: biostudies-literature
Moeller Arne A Zhao Chunxia C Fried Michael G MG Wilson-Kubalek Elizabeth M EM Carragher Bridget B Whiteheart Sidney W SW
Journal of structural biology 20120105 2
Homohexameric, N-Ethylmaleimide Sensitive Factor (NSF) disassembles Soluble NSF Attachment Protein Receptor (SNARE) complexes after membrane fusion, an essential step in vesicular trafficking. NSF contains three domains (NSF-N, NSF-D1, and NSF-D2), each contributing to activity. We combined electron microscopic (EM) analysis, analytical ultracentrifugation (AU) and functional mutagenesis to visualize NSF's ATPase cycle. 3D density maps show that NSF-D2 remains stable, whereas NSF-N undergoes lar ...[more]