Ontology highlight
ABSTRACT:
SUBMITTER: Vivona S
PROVIDER: S-EPMC3750193 | biostudies-literature | 2013 Aug
REPOSITORIES: biostudies-literature
The Journal of biological chemistry 20130708 34
Vesicle trafficking in eukaryotic cells is facilitated by SNARE-mediated membrane fusion. The ATPase NSF (N-ethylmaleimide-sensitive factor) and the adaptor protein α-SNAP (soluble NSF attachment protein) disassemble all SNARE complexes formed throughout different pathways, but the effect of SNARE sequence and domain variation on the poorly understood disassembly mechanism is unknown. By measuring SNARE-stimulated ATP hydrolysis rates, Michaelis-Menten constants for disassembly, and SNAP-SNARE b ...[more]