Association of matrix Gla protein gene allelic polymorphisms (G(-7)→A, T(-138)→C and Thr(83)→Ala) with acute coronary syndrome in the Ukrainian population.

ABSTRACT:

Background

Several allelic variants of matrix γ-carboxyglutamic acid protein (MGP) can differentially affect the development of certain forms of ischemic heart disease depending on specific characteristics of each population.

Objective

To study the distribution of allelic variants of MGP promoter T(-138)→C (rs1800802) and G(-7)→A (rs1800801), and Thr(83)→Ala exon 4 (rs4236) polymorphisms in a Ukrainian population of patients with acute coronary syndrome (ACS).

Methods

Polymerase chain reaction and restriction fragment length polymorphism (RFLP) analysis were used to detect the above-mentioned variants of the MGP gene in 115 patients with ACS and in 140 essentially healthy individuals.

Results

The distribution of homozygous carriers of a major allelic variant, and heterozygous and homozygous minor allele variants of the T(-138)→C MGP promoter polymorphism in patients with ACS were 59.8%, 32.7% and 7.5%, respectively. The corresponding distributions of variants in the control group were 54.0%, 41.0% and 5.0%, respectively (P>0.05 [χ(2) test]). With respect to the G(-7)→A polymorphism, the respective distributions were 42.1%, 45.6% and 12.3%, compared with 50.7%, 45.0% and 4.3% in the control group, respectively (P<0.05). Finally, the respective distributions according to the Thr(83)→Ala exon 4 polymorphism were 42.6%, 43.5% and 13.9%, respectively, compared with 45.3%, 43.0% and 11.7% in the control group. Using logistic regression analysis, it was estimated that the A/A genotype (G(-7)→A polymorphism) was significantly (P=0.02) associated with ACS (OR 4.302 [95% CI 1.262 to 14.673]).

Conclusion

The allelic A/A promoter variant of MGP G(-7)→A polymorphism can be considered a risk factor for ACS in the Ukrainian population.

SUBMITTER: Garbuzova VY 

PROVIDER: S-EPMC3383365 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature