Ontology highlight
ABSTRACT:
SUBMITTER: Mukherjee S
PROVIDER: S-EPMC3383595 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Mukherjee Suranjana S Diaz Valencia J Daniel JD Stewman Shannon S Metz Jeremy J Monnier Sylvain S Rath Uttama U Asenjo Ana B AB Charafeddine Rabab A RA Sosa Hernando J HJ Ross Jennifer L JL Ma Ao A Sharp David J DJ
Cell cycle (Georgetown, Tex.) 20120615 12
Fidgetin is a member of the AAA protein superfamily with important roles in mammalian development. Here we show that human Fidgetin is a potent microtubule severing and depolymerizing the enzyme used to regulate mitotic spindle architecture, dynamics and anaphase A. In vitro, recombinant human Fidgetin severs taxol-stabilized microtubules along their length and promotes depolymerization, primarily from their minus-ends. In cells, human Fidgetin targets to centrosomes, and its depletion with siRN ...[more]