Ontology highlight
ABSTRACT:
SUBMITTER: Rosenblum G
PROVIDER: S-EPMC3384345 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Rosenblum Gabriel G Chen Chunlai C Kaur Jaskiran J Cui Xiaonan X Goldman Yale E YE Cooperman Barry S BS
Nucleic acids research 20120314 12
We present a flexible, real-time-coupled transcription-translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along with numerical simulation of a reaction kinetics model, the assay permits quantitative estimation of the effects on full-length protein synthesis of various additions, subtractions or substitutions to the protein synthesis machinery. Since the assay uses continuous fluorescence monitoring, it is much simpler and more rapid than other assays ...[more]