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Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates.

ABSTRACT: Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its expression under the degradation conditions of diethylphthalate (DEP). Pyranose oxidase expression was enhanced up to 30% by the addition of DEP, and this result supports the possible involvement of pyranose oxidase in the degradation of recalcitrant compounds.

SUBMITTER: Kim B

PROVIDER: S-EPMC3385089 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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Enhanced Expression of Glucose 2-Oxidase in Phlebia tremellosa by Addition of Phthalates.

Kim Baikjoong B Kim Hyewon H Choi Hyoung T HT

Mycobiology 20110323 1

Most fungi possess several hydrogen peroxide-generating enzymes, glucose oxidase and pyranose oxidase. Pyranose oxidase can use glucose as its substrate to generate hydrogen peroxide. White rot fungi, which degrade diverse recalcitrant compounds, contain lignin-degrading enzymes, and lignin peroxidase and manganese peroxidase require hydrogen peroxide for their enzymatic reactions. In this study, we isolated a cDNA fragment of pyranose oxidase from Phlebia tremellosa using PCR and examined its e ...[more]

PMID: 22783078

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